Lactoferrin (Lf) is an iron binding glycoprotein of transferrin family, present in almost all mammalian secretions and neutrophils. A variety of biological functions have been ascribed to Lf, including anti-inflammatory and immunomodulatory effects. The protective action of Lf during inflammation is related to its influence on cytokine production and capacity to bind potentially toxic iron at inflammation foci. In our previous studies performed in collaboration with the Department of Clinical Bacteriology, University of Goteborg, we found that LPS (lipopolysaccharide) -induced cytokine production, in particular TNF-a, IL-1β, IL-6, are inhibited by Lf. The binding of protein to LPS could partly explain its inhibitory activity since the effect could be seen when Lf was added before as well after cell stimulation. This suggests that other mechanism(s) than simple LPS scavenging property of Lf is (are) responsible for the inhibitory activity.
In this respect, our group approaches two aspects:
The mode of action of Lf on intracellular signal transduction pathways.
Our preliminary results showed that Lf is taken up by monocytic cells. Therefore it is possible that Lf, following the binding to the cells, might affect some intracellular pathways involved in the cell response to external stimuli such as bacterial endotoxins. LPS stimulation of monocytes has been shown to induce the activation of three subgroups of MAPKinase familly-p38, ERK1/2 and JNK. These enzymes play an important role in the control of proinflammatory cytokine production mediated by LPS. We propose to study the effect of Lf on the phosphorylation and activation of MAPKinases in the presence/absence of LPS. The results will be related to the effect of Lf on the LPS-induced cytokine production in monocytic THP-1 cells. This aspect involves collaboration with Dr. Inger Mattsby-Baltzer group from the Department of Clinical Bacteriology, University of Gothenburg, Sweden.
The anti-inflammatory properties of Lf in vivo: use of liposomes as possible carriers for Lf in the treatment of Rheumatoid Arthritis.
Exhibiting antimicrobial and anti-inflammatory activities Lf could have therapeutic potential in arthritic disease. We are investigating the ability of free and liposome-entrapped Lf to reduce inflammation when administrated to the joint. Current studies aim to establish the suitable liposomal formulation able to deliver Lf efficiently into the joint of mice with collagen-induced rheumatoid arthritis.Liposomes-entrapped Lf is expected to serve as a better tool and therapeutic agent against inflammation. This part is developed in collaboration with Dr. James Brewer group from the Department of Clinical Immunology, Glasgow University, UK, coordinated by Prof. F. Y. Liew.
The project should provide important information for understanding the anti-inflammatory activity of Lf in vivo.
In the next years our research will focus on the cellular and molecular mechanisms involved in the anti-inflammatory and immunomodulatory activities of Lf.
Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation, Coatings(9), 2019
Icriverzi M, Rusen L, Brajnicov S, Bonciu A, Dinescu M, Cimpean A, Evans RW, Dinca V, Roseanu A
Biocompatible pure ZnO nanoparticles-3D bacterial cellulose biointerfaces with antibacterial properties, Arabian Journal of Chemistry, 2018
Dinca V, Mocanu A, Isopencu G, Busuioc C, Brajnicov S, Vlad A, Icriverzi M, Roseanu A, Dinescu M, Stroescu M.
In vitro behavior of human mesenchymal stem cells on poly(N-isopropylacrylamide) based biointerfaces obtained by matrix assisted pulsed laser evaporation(Applied Surface Science), 2018
M. Icriverzi, L. Rusen, L. E. Sima, A. Moldovan, S. Brajnicov, A. Bonciu, N. Mihailescu, M. Dinescu, A. Cimpean, A. Roseanu, V. Dinca
Physical-chemical characterization and biological assessment of simple and lithium-doped biological-derived hydroxyapatite thin films for a new generation of metallic implants(Applied Surface Science), 2018
Popescu A., Florian P., Stan G., Popescu-Pelin G., Zgura I., Enculescu M., Oktar F., Trusca R., Sima L., Roseanu A
Diseases of Civilization - Cancer, Diabetes, Obesity and Acne - the Implication of Milk, IGF-1 and mTORC1., Maedica, 13(4), 273-281, 2018
Clatici VG, Voicu C, Voaides C, Roseanu A, Icriverzi M, Jurcoane S
Lipid Nanostructures Containing Atorvastatin Decrease Lipopolysaccharide-Induced Inflammation in Macrophages(Journal of Nanoscience and Nanotechnology), 2017
Moisei Magdalena, Craciunescu Oana, Moldovan Lucia, Roseanu Anca, Trif Mihaela
Modern Technologies for Creating the Thin-Film Systems and Coatings, 171-191, 2017
Rusen, L.; Dinca, V.; Mustaciosu, C.; Icriverzi, M.; Sima, L.E.; Bonciu, A.; Brajnicov, S.; Mihailescu, N.; Dumitrescu, N.; Popovici, A.I., Roseanu A.; Dinescu M
Novel Hydrogel-Advanced Modified Clay Nanocomposites as Possible Vehicles for Drug Delivery and Controlled Release., Nanomaterials (Basel, Switzerland), 7(12), 2017
Ianchis R, Ninciuleanu CM, Gifu IC, Alexandrescu E, Somoghi R, Gabor AR, Preda S, Nistor CL, Nitu S, Petcu C, Icriverzi M, Florian PE, Roseanu AM
Lead-Free Piezoelectric (Ba,Ca)(Zr,Ti)O3 Thin Films for Biocompatible and Flexible Devices, ACS applied materials & interfaces, 9(1), 266-278, 2017
Scarisoreanu ND, Craciun F, Ion V, Birjega R, Bercea A, Dinca V, Dinescu M, Sima LE, Icriverzi M, Roseanu A, Gruionu L, Gruionu G
Liposomes as Delivery System of Chondroitin Sulfate to the Arthritic Joint by Intra-articular Administration., Austin Arthritis, 1(3), 1011, 2016
Trif M and Craciunescu O
Optimization of Recombinant Lipoic Acid Ligase Expression from Bacterial Cells(Romanian Biotechnological Letters), 2016
Paula Ecaterina Florian, Georgiana Petrareanu, Simona Maria Ruţă, Anca Roseanu
Recent advances in human viruses imaging studies., Journal of basic microbiology, 56(6), 591-607, 2016
Florian PE, Rouillé Y, Ruta S, Nichita N, Roseanu A
Cytotoxicity and intracellular fate of PLGA and chitosan-coated PLGA nanoparticles in Madin-Darby bovine kidney (MDBK) and human colorectal adenocarcinoma (Colo 205) cells., Journal of biomedical materials research. Part A, 103(11), 3599-611, 2015
Trif M, Florian PE, Roseanu A, Moisei M, Craciunescu O, Astete CE, Sabliov CM
Liposomal formulation of chondroitin sulfate enhances its antioxidant and anti-inflammatory potential in L929 fibroblast cell line., Journal of liposome research, 23(2), 145-53, 2013
Craciunescu O, Moldovan L, Moisei M, Trif M
Liposomalization of lactoferrin enhanced its anti-tumoral effects on melanoma cells., Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 23(3), 485-92, 2010
Roseanu A, Florian PE, Moisei M, Sima LE, Evans RW, Trif M
Multifunctional thin films of lactoferrin for biochemical use deposited by MAPLE technique, Applied Surface Science, 255(10), 5491-5495, 2009
Constantinescu C, Palla-Papavlu A, Rotaru A, Florian P, Chelu F, Icriverzi M, Nedelcea A, Dinca V, Roseanu A. Dinescu M.
Designing lipid nanostructures for local delivery of biologically active macromolecules., Journal of liposome research, 17(3-4), 237-48, 2007
Trif M, Roseanu A, Brock JH, Brewer JM
Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases., Experimental biology and medicine (Maywood, N.J.), 226(6), 559-64, 2001
Trif M, Guillen C, Vaughan DM, Telfer JM, Brewer JM, Roseanu A, Brock JH
Intarirea capacitatii administrative a Institutului de Biochimie
Project director: Anca Roseanu
Începând cu data de 16.06.2010, Institutul de Biochimie beneficiază de asistenţă financiară nerambursabilă pentru implementarea proiectului “Întărirea capacităţii administrative”, în baza contractului de finanţare nr. 167/16.06.2010 semnat cu Organismul Intermediar ANCS Bucureşti.