Romanian Academy
Stefan Szedlacsek, Dr.
Group: Enzymology
Department: EnzymologyHead of Department
Research interests: TBA, Researcher | Teaching staff | Scientific reviewer | Manager | Consultant.
Currently working on
Institute of Biochemistry of the Romanian Academy , Bucharest.
Department of Enzymology .
Department of Enzymology .
Biography
Professor Dr. Stefan Eugen Szedlacsek is the Head of the Enzymology Department at the Institute of Biochemistry of the Romanian Academy. He holds a PhD degree in Biotechnology from Polytechnic University of Bucharest as well as a MSc in Organic Synthesis (Polytechnic University- Bucharest) and MSc in Mathematics (Bucharest University). As a visiting scientist, he performed research in the field of cholesterol metabolism University of Illinois at Urbana-Champaign (USA), where he succeeded to evidence a new pathway in the metabolism of oxysterols. He is an “Alexander von Humboldt“ fellow and worked in Germany, in the Institute of Biochemistry (Kiel University), Max-Plack Institute for Physiological Chemistry (Dortmund) and Max-Planck Institute for Biophysics (Frankfurt) where he solved the crystal structure of PTP-SL/PTPBR7 catalytic domain, the first structure of a KIM-containing Protein Tyrosine Phosphatases PTP), and demonstrated the lateral dimerization of extracellular region of receptor-like PTP μ. Currently, Professor Szedlacsek and his team studies structure-function relationship in enzymes involved in cellular signaling. He has supervised numerous PhD and master’s theses. Together with his group, he participated as Principal Investigator/Group Leader in the Marie Curie Research Training Networks - PTPNET; MRTN-CT- 2006-035830 (FP6), financed by the European Community. Also, he received a „P-CUBE TNA Project” Fellowship (FP 7- EU) from Oxford University (United Kingdom). Professor Szedlacsek was Project Coordinator for research consortia within a number of projects financed by the Romanian Research Authority.
Papers
- . Regulation of TRPM8 channel activity by Src-mediated tyrosine phosphorylation. Journal of Cellular Physiology, 2020, 235(6):5192-5203.IF=4.52
- . Biological and molecular modifications induced by cadmium and arsenic during breast and prostate cancer development. Environ Res, 2019, 178:108700.IF=5.03
- . Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation. International Journal of Molecular Sciences, 2019, 20(24):6307.IF=4.18
- . Crystal structure of a xylulose 5-phosphate phosphoketolase. Insights into the substrate specificity for xylulose 5-phosphate. Journal of Structural Biology, 2019, 207(1):85-102.IF=3.75
- . Collagen regulates the ability of endothelial progenitor cells to protect hypoxic myocardium through a mechanism involving miR-377/VE-PTP axis. Journal of cellular and molecular medicine, 2018, 22(10):4700-4708.IF=4.66
- . WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton. Scientific reports, 2018, 8(1):2910.
- . Expression, Purification, and Kinetic Analysis of PTP Domains. Methods in molecular biology (Clifton, N.J.), 2016, 1447:39-66.
- . Phosphoketolases from Lactococcus lactis, Leuconostoc mesenteroides and Pseudomonas aeruginosa: Dissimilar sequences, similar substrates but distinct enzymatic characteristics. Applied Microbiology and Biotechnology, 2014, 18(98):7855-67.IF=3.34
- . Analysis of molecular determinants of PRL-3. Journal of cellular and molecular medicine, 2009, 13(9B):3141-50.
Grants
Reconstruction of Ancestor of Receptor Protein Tyrosine Phosphatase Catalytic Domain
2011-2016
Acronym: Contract: 296/2011, Cod Depunere: PN-II-ID-PCE-2011-3-0743
Project director: Stefan Szedlacsek
Synthesis of the ancestral PTP catalytic domain and its characterization both in vitro and in vivo.
Compound for inhibition of certain signaling processes related to the evolution of the cognitive processes
2017-2018
Acronym: Ctr.327/27.03.2017/Company CRU SRL, Medical Services Company/ Dunakesz, Hungary
Project director: Stefan Szedlacsek
Molecular modeling of a set of peptides that can disrupt the GluA2-Cterm complex with STEP (Complex A) or BRAG2 (ComplexB).
Novel radiolabeled affibodies for targeted imaging and therapy
2019-2021
Acronym: Acord bilateral nr.3698/13.09.2018 Academia Romana- Academia Ungara de Stiinte
Project director: Stefan Szedlacsek
The project is agreed as a joint collaboration among IBAR and ATOMKI, UD and IFIN-HH is a cost free participant. There are two main directions envisaged by the proposed project: receptors mapping and therapy, using an affibody against HER2 receptor, combined with an adequate radioisotope. In this respect, the specific objectives are: a) expression and purification of affibodies; b) establish labeling procedures; c) ex vivo and/or in vivo testing of optimal compounds.
Structure, Regulation, and Biological Function
2007-2011
Acronym: PTPNET
Project director: Stefan Szedlacsek
PTPs are proteins with enzymatic properties and a range of cell and tissue functions.