Reconstruction of Ancestor of Receptor Protein Tyrosine Phosphatase Catalytic

Reconstruction of Ancestor of Receptor Protein Tyrosine Phosphatase Catalytic 2011 - 2016
Acronym: PTPs
Project director: Stefan Szedlacsek
PN-II-ID-PCE-2011-3-0743

Proteins are essential players of all biological processes and they are involved in practically every function performed by a living cell.

The present project aims to synthesize the ancestral catalytic domain of PTPs as corresponding to the derived sequence, to characterize it by a multitude of in vivo and in vitro procedures and to offer in this way new information regarding the evolution of the PTPs and their role in the ancient world.

We expect that the sequence of the ancestor PTP together with its enzyme kinetic characteristics, in vitro and in vivo substrate specificity profiles will have a major impact on understanding how PTPs evolved from an ancestral form to the existing representatives.

Stefan Szedlacsek, Dr.
Stefan Szedlacsek, Dr.

Head of Department

Prof. Dr. Stefan Szedlacsek is the Head of the Enzymology Department at the Institute of Biochemistry of the Romanian Academy. He holds a PhD degree in Biotechnology from Polytechnical University of Bucharest as well as a MSc in Organic Synthesis (Polytechnical University, Bucharest) and MSc in Mathematics (University of Bucharest). As a visiting scientist, he performed research in the field of cholesterol metabolism at the University of Illinois at Urbana-Champaign (USA), where he succeeded to evidence a new pathway in the metabolism of oxysterols.  He is an “Alexander von Humboldt” fellow and worked in Germany, in the Institute of Biochem More...

  1. Synthesis of an ancestor of receptor protein tyrosine phosphatase catalytic domain (PTPAD1). Preparation of mutant and truncated forms of PTP-AD1;
  2. Enzyme kinetic characterization of PTP-AD1 and derived forms on a synthetic PTP substrate;
  3. Evaluation of substrate specificity of PTP-AD1 and its derived forms using microarray profiling on pTyr-containing peptides;
  4. Evaluation of catalytic efficiency and substrate specificity of PTP-AD1 and its  derived forms on cellular pTyr-containing proteins.

Dr. Stefan Szedlacsek, Romanian Head of Project

Postdoctoral researcher: Mihaela Mențel

Postdoctoral researcher: Georgiana Petrăreanu

PhD Student: Rodica-Aura Badea

Msc: Aura-Elena Ionescu

Student: Ioana Iancu

Under construction

 

Articles:

(1) Böhmer F, Szedlacsek S, Tabernero L, Ostman A, den Hertog J., “Protein tyrosine phosphatase structure-function relationships in regulation and pathogenesis”. FEBS J. 2013 Jan; 280(2):413-31. doi: 10.1111/j.1742-4658.2012.08655.x.; (2012) 

(2) Petrareanu G., “The molecular and cellular characterization of a non-receptor protein tyrosine phosphatase”, Rom. J. Biochem., 50, (2013); 

(3) Mentel M., Iancu I., Kibedi Szabo C. Z., Munteanu C.V.A., Szedlacsek S., “Co-expression of human wdr1 gene with a chaperone increases its protein solubility”, Rom. J. Biochem., 50, 1, 39–51 (2013)

Conference: 

Mentel M., Iancu I., Ionescu A.E., Kibedi Szabo C. Z., Munteanu C.V.A., Szedlacsek S.; "Identification of highly efficient substrate-trapping mutant of eyes absent tyrosine phosphatase”, Oral presentation at International Conference of RSBMB, June 5-6, 2014, Baile Felix, Oradea, Romania (2014)

Poster presentations:

(1) “Effects of site-directed mutagenesis on the enzymatic activity of non-receptor protein tyrosine phosphatase PTPD1” (Meeting abstracts-poster); FEBS Journal Special Issue: 22nd IUBMB & 37th FEBS Congress, Seville, 2012; ISSN/ISBN 1742-464X

(2) “Receptor-like protein tyrosine phosphatase mu dephosphorylates and interacts with phospholipase C gamma 2” (Meeting abstract-poster); FEBS Journal Special Issue: 22nd IUBMB & 37th FEBS Congress, Seville, 2012; ISSN/ISBN 1742-464X

(3) “Identification of potential phosphorylation sites of actin-interacting protein 1” (Meeting abstract-poster) FEBS Journal Special Issue: 22nd IUBMB & 37th FEBS Congress, Seville, 2012; ISSN/ISBN 1742-464X;

(4) “The presentation of a poster entitled „Mass Spectrometric investigations regarding phosphorylation of human Eya3 by Src kinase”  (Meeting abstract-poster); Europhosphatase; Phosphorylation switches and cellular homeostasis; Authors: Aura Elena Ionescu, Mihaela Menţel, Cristian V. A. Munteanu, Cristian Marian Butnaru, Rodica Aura Badea, Ioana Iancu, Ştefan Eugen Szedlacsek), at the EMBO Conference - Turku, Finland; 2015