In the Instiute of Biochemistry, we have a longstanding experience in the use of flow cytometry (FACS) technology that was implemented in our institution starting 2006.
In the Instiute of Biochemistry, we have a longstanding experience in the use of flow cytometry (FACS) technology that was implemented in our institution starting 2006, with the acquisition of the BD FACSCalibur 1-laser instrument. The PROCERA project allowed the expansion of our technical capacity with the investment in two more machines: the BD FACSVerse 3-laser analyser and the BD FACSAria III 5-laser cell sorter.
Our expertise and instruments enable us to perform most flow cytometric assays that have been described in the literature. These range from routine cell cycle and proliferation analyses to immunophenotyping and multicoulour applications. Also, separation of subpopulations of cells is possible using our cell sorter or Miltenyi MACS magnetic-beads separation system. The technological workflow consists of staining, data collection, quality control, data analysis/interpretation, and creation of graphics.
Available applications are:
1-laser (488 nm)
Cell QuestPro software analyser
3-lasers (405, 488, 632 nm)
automated laser alignment
Volumetric Flow Sensor - cell absolut counting standard and high sensitivity modes
Universal loader - automatic acquisition from tube racks or plates
BD FACSuite software
FACSAriaIII cell sorter
5-lasers (355/405, 488, 561, 633 nm)
up to 18 colours
gel-coupled cuvette flow cell
70, 85, and 100 microns nozzles
up to 70000 events per second
four-way and single-cell sorting capabilities
automated cell deposition unit (ACDU) for slide and
plate sorting: 6-, 24-, 48-, 96- and 384-well plates
BD FACSDiva software
If you are a first time user or are reserving time on the FACSCalibur, FACSVerse or FACSAriaIII (for sorting or multi-color analysis), contact Dr. Livia Sima (021-2239069, room 322) with the appointment request. If you are a trained user, please feel free to use the scheduler available for each instrument.
To schedule a session of assisted acquisition and/or analysis, a minimum one week advance notice is required.
Online resources (blogs):
Main vendors of FACS reagents and kits:
A list of our publications:
Mihaela Uta, Livia Elena Sima, Patrik Hoffmann, Valentina Dinca, Norica Branza-Nichita, Development of a DsRed-expressing HepaRG cell line for real-time monitoring of hepatocyte-like cell differentiation by fluorescence imaging, with application in screening of novel geometric microstructured cell growth substrates, Biomedical microdevices 19 (1), 3 (2017)
Corotchi MC, Popa MA, Remes A, Sima LE, Gussi I, Plesu ML. " Isolation method and xeno-free culture conditions influence multipotent differentiation capacity of human Wharton.s jelly-derived mesenchymal stem cells., Stem Cell Res.Ther., 4, 81 (2013)
Dinca V, Florian PE, Sima LE, Rusen L, Constantinescu C, Evans RE, Dinescu M, Roseanu A " MAPLE-based method to obtain biodegradable hybrid polymeric thin films with embedded antitumoral agents.., Biomed.Microdev., DOI 10.1007/s10544-013-9801-9, July (2013)
Ivan V, Martinez-Sanchez E, Sima LE, Ooschot V, Klumperman J, Petrescu SM, Van der Sluj P."AP-3 and Rabip4' Coordinately Regulate Spatial Distribution of Lysosomes.", PLoS One., 7(10), e48142 (2012)/p>
Filimon A, Sima LE, Appelhans D, Voit B, Negroiu G."Internalization and Intracellular Trafficking of Poly(propylene imine) Glycodendrimers with Maltose Shell in Melanoma Cells.", Curr.Med.Chem., 19(29), 4955-4968 (2012)
Florian P, Macovei A, Sima LE, Nichita N, Mattsby-Baltzer I, Roseanu A."Endocytosis and trafficking of human lactoferrin in macrophage-like human THP-1 cells.", Biochem Cell Biol., 90(3), 449-455 (2012)
Sima LE, Stan GE, Morosanu CO, Melinescu A, Ianculescu A, Melinte R, Neamtu J, Petrescu SM "Differentiation of mesenchymal stem cells onto highly adherent radio frequency-sputtered carbonated hydroxylapatite thin films.", J Biomed Mater Res A, 95(4), 1203-1214 (2010)
Roseanu A, Florian PE, Moisei M, Sima LE, Evans RW, Trif M "Liposomalization of lactoferrin enhanced its anti-tumoral effects on melanoma cells.", Biometals, 23(3), 485-492 (2010)
Negroiu G, Piticescu RM, Chitanu GC, Mihailescu IN, Zdrentu L, Miroiu M "Biocompatibility evaluation of a novel hydroxyapatite-polymer coating for medical implants (in vitro tests)",J.Mat.Sci:Mat.Med, 19, 1537-1544 (2008)