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RECONSTRUCTION OF AN ANCESTOR OF RECEPTOR PROTEIN TYROSINE PHOSPHATASE CATALYTIC DOMAIN

RECONSTRUCTION OF AN ANCESTOR OF RECEPTOR PROTEIN TYROSINE PHOSPHATASE CATALYTIC DOMAIN 2011-2016
Acronym: Ctr.296/05.10.2011
Budget: 1.499.000 Ron
Project director: Stefan Szedlacsek
PN-II-ID-PCE-2011-3-0743

Synthesis of the catalytic domain of ancestral PTP and its characterization both in vitro and in vivo

RECONSTRUCTION OF AN ANCESTOR OF RECEPTOR PROTEIN TYROSINE PHOSPHATASE CATALYTIC DOMAIN

The project and the team aimed to identify the characterization of the catalytic domain of protein tyrosine ancestral phosphatase.

-Obtaining the sequences, but also the proteins corresponding to the ancestral catalytic domain, of the C-terminal truncated constructs and of the mutant forms.

-Determination of kinetic parameters on artificial substrate, as well as on phosphorylated tyrosine peptides

-Evaluation of substrate specificity on a library of phosphorylated tyrosine peptides for the most representative constructs

-Identification of consensus sequences indicating the dephosphorylation preferences of PTP-AD1 proteins

-The effect of PTP-AD1 on cellular phosphoproteins

Stefan Szedlacsek, Dr.
Stefan Szedlacsek, Dr.

Head of Department

Professor Dr. Stefan Eugen Szedlacsek is the Head of the Enzymology Department at the Institute of Biochemistry of the Romanian Academy. He holds a PhD degree in Biotechnology from Polytechnic University of Bucharest as well as a MSc in Organic Synthesis (Polytechnic University- Bucharest) and MSc in Mathematics (Bucharest University). As a visiting scientist, he performed research in the field of cholesterol metabolism University of Illinois at Urbana-Champaign (USA), where he succeeded to evidence a new pathway in the metabolism of oxysterols. He is an “Alexander von Humboldt“ fellow and worked in Germany, in the Institute of Biochemistry (Kiel University) More...

Aura-Elena Ionescu, PhD student
Aura-Elena Ionescu, PhD student

Scientific Researcher

Born: 19th of November 1988, Ploiesti, Romania 2003 - 2007: „Ion Luca Caragiale” National College, Ploieşti, Romania 2007- 2012: Faculty of Pharmacy at University of Medicine and Pharmacy "Carol Davila" Bucharest 2012 More...

Rodica-Aura Badea, Dr.
Rodica-Aura Badea, Dr.

Scientific Researcher

Rodica-Aura Badea is a researcher in the Institute of Biochemistry of the Romanian Academy. Rodica-Aura is currently working in Enzymology in the Enzymology.