Synthesis of the catalytic domain of ancestral PTP and its characterization both in vitro and in vivo
The project and the team aimed to identify the characterization of the catalytic domain of protein tyrosine ancestral phosphatase.
-Obtaining the sequences, but also the proteins corresponding to the ancestral catalytic domain, of the C-terminal truncated constructs and of the mutant forms.
-Determination of kinetic parameters on artificial substrate, as well as on phosphorylated tyrosine peptides
-Evaluation of substrate specificity on a library of phosphorylated tyrosine peptides for the most representative constructs
-Identification of consensus sequences indicating the dephosphorylation preferences of PTP-AD1 proteins
-The effect of PTP-AD1 on cellular phosphoproteins