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” Reconstruction of Ancestor of Receptor Protein Tyrosine Phosphatase Catalytic Domain ”

” Reconstruction of Ancestor of Receptor Protein Tyrosine Phosphatase Catalytic Domain ” 2011-2016
Acronym: Contract: 296/2011, Cod Depunere: PN-II-ID-PCE-2011-3-0743
Project director: Stefan Szedlacsek
External website: full link

Synthesis of the ancestral PTP catalytic domain and its characterization both in vitro and in vivo.

Receptor protein tyrosine phosphatases (RPTPs) have a pivotal role in regulating both normal and pathological cellular processes. Most RPTPs have two homologous intracellular domains but only the membrane proximal one is catalytically active. It has been broadly accepted that during evolution a hypothetical ancestral PTP domain duplicated leading to extant RPTPs. We propose in this project to reconstruct and characterize the catalytic domain of an ancestor of RPTPs. Initially, the ancestor gene will be synthesized then coded protein will be expressed in a prokaryotic system and purified to high purity. Similarly, several truncated and mutant forms will be obtained. The catalytic efficiency and substrate specificity under in vitro and in vivo conditions for all constructs will be evaluated and compared. It is expected that sequence of the ancestor PTP catalytic domain and its characteristics will consistently contribute to our understanding of the evolution of PTPs and the physiological function of the ancestor PTP. In addition, new insights into the role played by the molecular determinants of PTPs’ catalytic efficiency and substrate specificity will be generated. We expect that our results will help designing PTPs with defined substrate specificity.

Stefan Szedlacsek, Dr.
Stefan Szedlacsek, Dr.

Head of Department

Professor Dr. Stefan Eugen Szedlacsek is the Head of the Enzymology Department at the Institute of Biochemistry of the Romanian Academy. He holds a PhD degree in Biotechnology from Polytechnic University of Bucharest as well as a MSc in Organic Synthesis (Polytechnic University- Bucharest) and MSc in Mathematics (Bucharest University). As a visiting scientist, he performed research in the field of cholesterol metabolism University of Illinois at Urbana-Champaign (USA), where he succeeded to evidence a new pathway in the metabolism of oxysterols. He is an “Alexander von Humboldt“ fellow and worked in Germany, in the Institute of Biochemistry (Kiel University) More...

Aura-Elena Ionescu, PhD student
Aura-Elena Ionescu, PhD student

Scientific Researcher

Born: 19th of November 1988, Ploiesti, Romania 2003 - 2007: „Ion Luca Caragiale” National College, Ploieşti, Romania 2007- 2012: Faculty of Pharmacy at University of Medicine and Pharmacy "Carol Davila" Bucharest 2012 More...

Rodica-Aura Badea, Dr.
Rodica-Aura Badea, Dr.

Scientific Researcher

Rodica-Aura Badea is a researcher in the Institute of Biochemistry of the Romanian Academy. Rodica-Aura is currently working in Enzymology in the Enzymology.